Vitamin C enhances the generation of mouse and human induced pluripotent stem cells

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt

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Vitamin C enhances the generation of mouse and human induced pluripotent stem cells. / Esteban, Miguel Angel; Wang, Tao; Qin, Baoming; Yang, Jiayin; Qin, Dajiang; Cai, Jinglei; Li, Wen; Weng, Zhihui; Chen, Jiekai; Ni, Su; Chen, Keshi; Li, Yuan; Liu, Xiaopeng; Xu, Jianyong; Zhang, Shiqiang; Li, Feng; He, Wenzhi; Labuda, Krystyna; Song, Yancheng; Peterbauer, Anja; Wolbank, Susanne; Redl, Heinz; Zhong, Mei; Cai, Daozhang; Zeng, Lingwen; Pei, Duanqing.

I: Cell Stem Cell, Bind 6, Nr. 1, 08.01.2010, s. 71-9.

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt

Harvard

Esteban, MA, Wang, T, Qin, B, Yang, J, Qin, D, Cai, J, Li, W, Weng, Z, Chen, J, Ni, S, Chen, K, Li, Y, Liu, X, Xu, J, Zhang, S, Li, F, He, W, Labuda, K, Song, Y, Peterbauer, A, Wolbank, S, Redl, H, Zhong, M, Cai, D, Zeng, L & Pei, D 2010, 'Vitamin C enhances the generation of mouse and human induced pluripotent stem cells', Cell Stem Cell, bind 6, nr. 1, s. 71-9. https://doi.org/10.1016/j.stem.2009.12.001

APA

Esteban, M. A., Wang, T., Qin, B., Yang, J., Qin, D., Cai, J., Li, W., Weng, Z., Chen, J., Ni, S., Chen, K., Li, Y., Liu, X., Xu, J., Zhang, S., Li, F., He, W., Labuda, K., Song, Y., ... Pei, D. (2010). Vitamin C enhances the generation of mouse and human induced pluripotent stem cells. Cell Stem Cell, 6(1), 71-9. https://doi.org/10.1016/j.stem.2009.12.001

Vancouver

Esteban MA, Wang T, Qin B, Yang J, Qin D, Cai J o.a. Vitamin C enhances the generation of mouse and human induced pluripotent stem cells. Cell Stem Cell. 2010 jan. 8;6(1):71-9. https://doi.org/10.1016/j.stem.2009.12.001

Author

Esteban, Miguel Angel ; Wang, Tao ; Qin, Baoming ; Yang, Jiayin ; Qin, Dajiang ; Cai, Jinglei ; Li, Wen ; Weng, Zhihui ; Chen, Jiekai ; Ni, Su ; Chen, Keshi ; Li, Yuan ; Liu, Xiaopeng ; Xu, Jianyong ; Zhang, Shiqiang ; Li, Feng ; He, Wenzhi ; Labuda, Krystyna ; Song, Yancheng ; Peterbauer, Anja ; Wolbank, Susanne ; Redl, Heinz ; Zhong, Mei ; Cai, Daozhang ; Zeng, Lingwen ; Pei, Duanqing. / Vitamin C enhances the generation of mouse and human induced pluripotent stem cells. I: Cell Stem Cell. 2010 ; Bind 6, Nr. 1. s. 71-9.

Bibtex

@article{83cf3d34806c47ef905de9df82e506e6,
title = "Vitamin C enhances the generation of mouse and human induced pluripotent stem cells",
abstract = "Somatic cells can be reprogrammed into induced pluripotent stem cells (iPSCs) by defined factors. However, the low efficiency and slow kinetics of the reprogramming process have hampered progress with this technology. Here we report that a natural compound, vitamin C (Vc), enhances iPSC generation from both mouse and human somatic cells. Vc acts at least in part by alleviating cell senescence, a recently identified roadblock for reprogramming. In addition, Vc accelerates gene expression changes and promotes the transition of pre-iPSC colonies to a fully reprogrammed state. Our results therefore highlight a straightforward method for improving the speed and efficiency of iPSC generation and provide additional insights into the mechanistic basis of the reprogramming process.",
keywords = "Animals, Ascorbic Acid/pharmacology, Cells, Cultured, Cellular Reprogramming/drug effects, Cellular Senescence/drug effects, Cytological Techniques, Embryo, Mammalian/cytology, Fibroblasts/cytology, Humans, Induced Pluripotent Stem Cells/cytology, Mice",
author = "Esteban, {Miguel Angel} and Tao Wang and Baoming Qin and Jiayin Yang and Dajiang Qin and Jinglei Cai and Wen Li and Zhihui Weng and Jiekai Chen and Su Ni and Keshi Chen and Yuan Li and Xiaopeng Liu and Jianyong Xu and Shiqiang Zhang and Feng Li and Wenzhi He and Krystyna Labuda and Yancheng Song and Anja Peterbauer and Susanne Wolbank and Heinz Redl and Mei Zhong and Daozhang Cai and Lingwen Zeng and Duanqing Pei",
note = "Copyright 2010 Elsevier Inc. All rights reserved.",
year = "2010",
month = jan,
day = "8",
doi = "10.1016/j.stem.2009.12.001",
language = "English",
volume = "6",
pages = "71--9",
journal = "Cell Stem Cell",
issn = "1934-5909",
publisher = "Cell Press",
number = "1",

}

RIS

TY - JOUR

T1 - Vitamin C enhances the generation of mouse and human induced pluripotent stem cells

AU - Esteban, Miguel Angel

AU - Wang, Tao

AU - Qin, Baoming

AU - Yang, Jiayin

AU - Qin, Dajiang

AU - Cai, Jinglei

AU - Li, Wen

AU - Weng, Zhihui

AU - Chen, Jiekai

AU - Ni, Su

AU - Chen, Keshi

AU - Li, Yuan

AU - Liu, Xiaopeng

AU - Xu, Jianyong

AU - Zhang, Shiqiang

AU - Li, Feng

AU - He, Wenzhi

AU - Labuda, Krystyna

AU - Song, Yancheng

AU - Peterbauer, Anja

AU - Wolbank, Susanne

AU - Redl, Heinz

AU - Zhong, Mei

AU - Cai, Daozhang

AU - Zeng, Lingwen

AU - Pei, Duanqing

N1 - Copyright 2010 Elsevier Inc. All rights reserved.

PY - 2010/1/8

Y1 - 2010/1/8

N2 - Somatic cells can be reprogrammed into induced pluripotent stem cells (iPSCs) by defined factors. However, the low efficiency and slow kinetics of the reprogramming process have hampered progress with this technology. Here we report that a natural compound, vitamin C (Vc), enhances iPSC generation from both mouse and human somatic cells. Vc acts at least in part by alleviating cell senescence, a recently identified roadblock for reprogramming. In addition, Vc accelerates gene expression changes and promotes the transition of pre-iPSC colonies to a fully reprogrammed state. Our results therefore highlight a straightforward method for improving the speed and efficiency of iPSC generation and provide additional insights into the mechanistic basis of the reprogramming process.

AB - Somatic cells can be reprogrammed into induced pluripotent stem cells (iPSCs) by defined factors. However, the low efficiency and slow kinetics of the reprogramming process have hampered progress with this technology. Here we report that a natural compound, vitamin C (Vc), enhances iPSC generation from both mouse and human somatic cells. Vc acts at least in part by alleviating cell senescence, a recently identified roadblock for reprogramming. In addition, Vc accelerates gene expression changes and promotes the transition of pre-iPSC colonies to a fully reprogrammed state. Our results therefore highlight a straightforward method for improving the speed and efficiency of iPSC generation and provide additional insights into the mechanistic basis of the reprogramming process.

KW - Animals

KW - Ascorbic Acid/pharmacology

KW - Cells, Cultured

KW - Cellular Reprogramming/drug effects

KW - Cellular Senescence/drug effects

KW - Cytological Techniques

KW - Embryo, Mammalian/cytology

KW - Fibroblasts/cytology

KW - Humans

KW - Induced Pluripotent Stem Cells/cytology

KW - Mice

U2 - 10.1016/j.stem.2009.12.001

DO - 10.1016/j.stem.2009.12.001

M3 - Journal article

C2 - 20036631

VL - 6

SP - 71

EP - 79

JO - Cell Stem Cell

JF - Cell Stem Cell

SN - 1934-5909

IS - 1

ER -

ID: 317433854