Subcellular localization- and fibre type-dependent utilization of muscle glycogen during heavy resistance exercise in elite power and Olympic weightlifters
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Aim: Glycogen particles are found in different subcellular localizations, which are utilized heterogeneously in different fibre types during endurance exercise. Although resistance exercise typically involves only a moderate use of mixed muscle glycogen, the hypothesis of the present study was that high-volume heavy-load resistance exercise would mediate a pattern of substantial glycogen depletion in specific subcellular localizations and fibre types. Methods: 10 male elite weightlifters performed resistance exercise consisting of four sets of five (4 × 5) repetitions at 75% of 1RM back squats, 4 × 5 at 75% of 1RM deadlifts and 4 × 12 at 65% of 1RM rear foot elevated split squats. Muscle biopsies (vastus lateralis) were obtained before and after the exercise session. The volumetric content of intermyofibrillar (between myofibrils), intramyofibrillar (within myofibrils) and subsarcolemmal glycogen was assessed by transmission electron microscopy. Results: After exercise, biochemically determined muscle glycogen decreased by 38 (31:45)%. Location-specific glycogen analyses revealed in type 1 fibres a large decrement in intermyofibrillar glycogen, but no or only minor changes in intramyofibrillar or subsarcolemmal glycogen. In type 2 fibres, large decrements in glycogen were observed in all subcellular localizations. Notably, a substantial fraction of the type 2 fibres demonstrated near-depleted levels of intramyofibrillar glycogen after the exercise session. Conclusion: Heavy resistance exercise mediates a substantial utilization of glycogen from all three subcellular localization in type 2 fibres, while mostly taxing intermyofibrillar glycogen stores in type 1 fibres. Thus, a better understanding of the impact of resistance training on myocellular metabolism and performance requires a focus on compartmentalized glycogen utilization.
Originalsprog | Engelsk |
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Artikelnummer | e13561 |
Tidsskrift | Acta Physiologica |
Vol/bind | 231 |
Udgave nummer | 2 |
ISSN | 1748-1708 |
DOI | |
Status | Udgivet - 2021 |
Bibliografisk note
Funding Information:
Conception or design of the work: RH, SL and JN. Acquisition, analysis or interpretation of data for the work: All authors. All authors have approved the final version of the manuscript. Experiments and image analysis were carried out at the Department of Sports Science and Clinical Biomechanics at the University of Southern Denmark, Odense, Denmark. We acknowledge the Core Facility for Integrated Microscopy, Faculty of Health and Medical Sciences, University of Copenhagen. This work was supported by the Danish Council for Independent Research [DFF – 1333‐00144 to JN].
Funding Information:
Conception or design of the work: RH, SL and JN. Acquisition, analysis or interpretation of data for the work: All authors. All authors have approved the final version of the manuscript. Experiments and image analysis were carried out at the Department of Sports Science and Clinical Biomechanics at the University of Southern Denmark, Odense, Denmark. We acknowledge the Core Facility for Integrated Microscopy, Faculty of Health and Medical Sciences, University of Copenhagen. This work was supported by the Danish Council for Independent Research [DFF ? 1333-00144 to JN].
Publisher Copyright:
© 2020 Scandinavian Physiological Society. Published by John Wiley & Sons Ltd
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