Regulation of glucose homeostasis and insulin action by ceramide acyl-chain length: A beneficial role for very long-chain sphingolipid species

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Regulation of glucose homeostasis and insulin action by ceramide acyl-chain length : A beneficial role for very long-chain sphingolipid species. / Montgomery, Magdalene K.; Brown, Simon H.J.; Lim, Xin Y.; Fiveash, Corrine E.; Osborne, Brenna; Bentley, Nicholas L.; Braude, Jeremy P.; Mitchell, Todd W.; Coster, Adelle C.F.; Don, Anthony S.; Cooney, Gregory J.; Schmitz-Peiffer, Carsten; Turner, Nigel.

I: Biochimica et Biophysica Acta - Molecular and Cell Biology of Lipids, Bind 1861, Nr. 11, 01.11.2016, s. 1828-1839.

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt

Harvard

Montgomery, MK, Brown, SHJ, Lim, XY, Fiveash, CE, Osborne, B, Bentley, NL, Braude, JP, Mitchell, TW, Coster, ACF, Don, AS, Cooney, GJ, Schmitz-Peiffer, C & Turner, N 2016, 'Regulation of glucose homeostasis and insulin action by ceramide acyl-chain length: A beneficial role for very long-chain sphingolipid species', Biochimica et Biophysica Acta - Molecular and Cell Biology of Lipids, bind 1861, nr. 11, s. 1828-1839. https://doi.org/10.1016/j.bbalip.2016.08.016

APA

Montgomery, M. K., Brown, S. H. J., Lim, X. Y., Fiveash, C. E., Osborne, B., Bentley, N. L., Braude, J. P., Mitchell, T. W., Coster, A. C. F., Don, A. S., Cooney, G. J., Schmitz-Peiffer, C., & Turner, N. (2016). Regulation of glucose homeostasis and insulin action by ceramide acyl-chain length: A beneficial role for very long-chain sphingolipid species. Biochimica et Biophysica Acta - Molecular and Cell Biology of Lipids, 1861(11), 1828-1839. https://doi.org/10.1016/j.bbalip.2016.08.016

Vancouver

Montgomery MK, Brown SHJ, Lim XY, Fiveash CE, Osborne B, Bentley NL o.a. Regulation of glucose homeostasis and insulin action by ceramide acyl-chain length: A beneficial role for very long-chain sphingolipid species. Biochimica et Biophysica Acta - Molecular and Cell Biology of Lipids. 2016 nov. 1;1861(11):1828-1839. https://doi.org/10.1016/j.bbalip.2016.08.016

Author

Montgomery, Magdalene K. ; Brown, Simon H.J. ; Lim, Xin Y. ; Fiveash, Corrine E. ; Osborne, Brenna ; Bentley, Nicholas L. ; Braude, Jeremy P. ; Mitchell, Todd W. ; Coster, Adelle C.F. ; Don, Anthony S. ; Cooney, Gregory J. ; Schmitz-Peiffer, Carsten ; Turner, Nigel. / Regulation of glucose homeostasis and insulin action by ceramide acyl-chain length : A beneficial role for very long-chain sphingolipid species. I: Biochimica et Biophysica Acta - Molecular and Cell Biology of Lipids. 2016 ; Bind 1861, Nr. 11. s. 1828-1839.

Bibtex

@article{b4e07dcf50634c3aacc141d865ef3227,
title = "Regulation of glucose homeostasis and insulin action by ceramide acyl-chain length: A beneficial role for very long-chain sphingolipid species",
abstract = "In a recent study, we showed that in response to high fat feeding C57BL/6, 129X1, DBA/2 and FVB/N mice all developed glucose intolerance, while BALB/c mice displayed minimal deterioration in glucose tolerance and insulin action. Lipidomic analysis of livers across these five strains has revealed marked strain-specific differences in ceramide (Cer) and sphingomyelin (SM) species with high-fat feeding; with increases in C16-C22 (long-chain) and reductions in C > 22 (very long-chain) Cer and SM species observed in the four strains that developed HFD-induced glucose intolerance. Intriguingly, the opposite pattern was observed in sphingolipid species in BALB/c mice. These strain-specific changes in sphingolipid acylation closely correlated with ceramide synthase 2 (CerS2) protein content and activity, with reduced CerS2 levels/activity observed in glucose intolerant strains and increased content in BALB/c mice. Overexpression of CerS2 in primary mouse hepatocytes induced a specific elevation in very long-chain Cer, but despite the overall increase in ceramide abundance, there was a substantial improvement in insulin signal transduction, as well as decreased ER stress and gluconeogenic markers. Overall our findings suggest that very long-chain sphingolipid species exhibit a protective role against the development of glucose intolerance and hepatic insulin resistance.",
keywords = "Ceramide species, Endoplasmic reticulum stress, Insulin sensitivity and resistance, Lipid metabolism, Lipidomics, Obesity",
author = "Montgomery, {Magdalene K.} and Brown, {Simon H.J.} and Lim, {Xin Y.} and Fiveash, {Corrine E.} and Brenna Osborne and Bentley, {Nicholas L.} and Braude, {Jeremy P.} and Mitchell, {Todd W.} and Coster, {Adelle C.F.} and Don, {Anthony S.} and Cooney, {Gregory J.} and Carsten Schmitz-Peiffer and Nigel Turner",
note = "Funding Information: We thank the Biological Testing Facility at the Garvan Institute and the UNSW Biological Resources Centre (Sydney, Australia) for assistance with animal care. This work was supported by funding from the National Health and Medical Research Council of Australia ( NHMRC - 535921 ), the Diabetes Australia Research Trust , the University of Wollongong and the Australian Research Council (ARC - LE0989078 ). MKM and GJC are supported by NHMRC Research Fellowships ( APP1071143 and APP1107290 ) and TWM and NT are supported by ARC Future Fellowships ( FT110100249 and FT120100371 ). Publisher Copyright: {\textcopyright} 2016 Elsevier B.V.",
year = "2016",
month = nov,
day = "1",
doi = "10.1016/j.bbalip.2016.08.016",
language = "English",
volume = "1861",
pages = "1828--1839",
journal = "B B A - Molecular and Cell Biology of Lipids",
issn = "1388-1981",
publisher = "Elsevier",
number = "11",

}

RIS

TY - JOUR

T1 - Regulation of glucose homeostasis and insulin action by ceramide acyl-chain length

T2 - A beneficial role for very long-chain sphingolipid species

AU - Montgomery, Magdalene K.

AU - Brown, Simon H.J.

AU - Lim, Xin Y.

AU - Fiveash, Corrine E.

AU - Osborne, Brenna

AU - Bentley, Nicholas L.

AU - Braude, Jeremy P.

AU - Mitchell, Todd W.

AU - Coster, Adelle C.F.

AU - Don, Anthony S.

AU - Cooney, Gregory J.

AU - Schmitz-Peiffer, Carsten

AU - Turner, Nigel

N1 - Funding Information: We thank the Biological Testing Facility at the Garvan Institute and the UNSW Biological Resources Centre (Sydney, Australia) for assistance with animal care. This work was supported by funding from the National Health and Medical Research Council of Australia ( NHMRC - 535921 ), the Diabetes Australia Research Trust , the University of Wollongong and the Australian Research Council (ARC - LE0989078 ). MKM and GJC are supported by NHMRC Research Fellowships ( APP1071143 and APP1107290 ) and TWM and NT are supported by ARC Future Fellowships ( FT110100249 and FT120100371 ). Publisher Copyright: © 2016 Elsevier B.V.

PY - 2016/11/1

Y1 - 2016/11/1

N2 - In a recent study, we showed that in response to high fat feeding C57BL/6, 129X1, DBA/2 and FVB/N mice all developed glucose intolerance, while BALB/c mice displayed minimal deterioration in glucose tolerance and insulin action. Lipidomic analysis of livers across these five strains has revealed marked strain-specific differences in ceramide (Cer) and sphingomyelin (SM) species with high-fat feeding; with increases in C16-C22 (long-chain) and reductions in C > 22 (very long-chain) Cer and SM species observed in the four strains that developed HFD-induced glucose intolerance. Intriguingly, the opposite pattern was observed in sphingolipid species in BALB/c mice. These strain-specific changes in sphingolipid acylation closely correlated with ceramide synthase 2 (CerS2) protein content and activity, with reduced CerS2 levels/activity observed in glucose intolerant strains and increased content in BALB/c mice. Overexpression of CerS2 in primary mouse hepatocytes induced a specific elevation in very long-chain Cer, but despite the overall increase in ceramide abundance, there was a substantial improvement in insulin signal transduction, as well as decreased ER stress and gluconeogenic markers. Overall our findings suggest that very long-chain sphingolipid species exhibit a protective role against the development of glucose intolerance and hepatic insulin resistance.

AB - In a recent study, we showed that in response to high fat feeding C57BL/6, 129X1, DBA/2 and FVB/N mice all developed glucose intolerance, while BALB/c mice displayed minimal deterioration in glucose tolerance and insulin action. Lipidomic analysis of livers across these five strains has revealed marked strain-specific differences in ceramide (Cer) and sphingomyelin (SM) species with high-fat feeding; with increases in C16-C22 (long-chain) and reductions in C > 22 (very long-chain) Cer and SM species observed in the four strains that developed HFD-induced glucose intolerance. Intriguingly, the opposite pattern was observed in sphingolipid species in BALB/c mice. These strain-specific changes in sphingolipid acylation closely correlated with ceramide synthase 2 (CerS2) protein content and activity, with reduced CerS2 levels/activity observed in glucose intolerant strains and increased content in BALB/c mice. Overexpression of CerS2 in primary mouse hepatocytes induced a specific elevation in very long-chain Cer, but despite the overall increase in ceramide abundance, there was a substantial improvement in insulin signal transduction, as well as decreased ER stress and gluconeogenic markers. Overall our findings suggest that very long-chain sphingolipid species exhibit a protective role against the development of glucose intolerance and hepatic insulin resistance.

KW - Ceramide species

KW - Endoplasmic reticulum stress

KW - Insulin sensitivity and resistance

KW - Lipid metabolism

KW - Lipidomics

KW - Obesity

UR - http://www.scopus.com/inward/record.url?scp=84987917179&partnerID=8YFLogxK

U2 - 10.1016/j.bbalip.2016.08.016

DO - 10.1016/j.bbalip.2016.08.016

M3 - Journal article

C2 - 27591968

AN - SCOPUS:84987917179

VL - 1861

SP - 1828

EP - 1839

JO - B B A - Molecular and Cell Biology of Lipids

JF - B B A - Molecular and Cell Biology of Lipids

SN - 1388-1981

IS - 11

ER -

ID: 322908791