Profiling Microglia From Alzheimer’s Disease Donors and Non-demented Elderly in Acute Human Postmortem Cortical Tissue

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Profiling Microglia From Alzheimer’s Disease Donors and Non-demented Elderly in Acute Human Postmortem Cortical Tissue. / Alsema, Astrid M.; Jiang, Qiong; Kracht, Laura; Gerrits, Emma; Dubbelaar, Marissa L.; Miedema, Anneke; Brouwer, Nieske; Hol, Elly M.; Middeldorp, Jinte; van Dijk, Roland; Woodbury, Maya; Wachter, Astrid; Xi, Simon; Möller, Thomas; Biber, Knut P.; Kooistra, Susanne M.; Boddeke, Erik W.G.M.; Eggen, Bart J.L.

I: Frontiers in Molecular Neuroscience, Bind 13, 134, 2020.

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt

Harvard

Alsema, AM, Jiang, Q, Kracht, L, Gerrits, E, Dubbelaar, ML, Miedema, A, Brouwer, N, Hol, EM, Middeldorp, J, van Dijk, R, Woodbury, M, Wachter, A, Xi, S, Möller, T, Biber, KP, Kooistra, SM, Boddeke, EWGM & Eggen, BJL 2020, 'Profiling Microglia From Alzheimer’s Disease Donors and Non-demented Elderly in Acute Human Postmortem Cortical Tissue', Frontiers in Molecular Neuroscience, bind 13, 134. https://doi.org/10.3389/fnmol.2020.00134

APA

Alsema, A. M., Jiang, Q., Kracht, L., Gerrits, E., Dubbelaar, M. L., Miedema, A., Brouwer, N., Hol, E. M., Middeldorp, J., van Dijk, R., Woodbury, M., Wachter, A., Xi, S., Möller, T., Biber, K. P., Kooistra, S. M., Boddeke, E. W. G. M., & Eggen, B. J. L. (2020). Profiling Microglia From Alzheimer’s Disease Donors and Non-demented Elderly in Acute Human Postmortem Cortical Tissue. Frontiers in Molecular Neuroscience, 13, [134]. https://doi.org/10.3389/fnmol.2020.00134

Vancouver

Alsema AM, Jiang Q, Kracht L, Gerrits E, Dubbelaar ML, Miedema A o.a. Profiling Microglia From Alzheimer’s Disease Donors and Non-demented Elderly in Acute Human Postmortem Cortical Tissue. Frontiers in Molecular Neuroscience. 2020;13. 134. https://doi.org/10.3389/fnmol.2020.00134

Author

Alsema, Astrid M. ; Jiang, Qiong ; Kracht, Laura ; Gerrits, Emma ; Dubbelaar, Marissa L. ; Miedema, Anneke ; Brouwer, Nieske ; Hol, Elly M. ; Middeldorp, Jinte ; van Dijk, Roland ; Woodbury, Maya ; Wachter, Astrid ; Xi, Simon ; Möller, Thomas ; Biber, Knut P. ; Kooistra, Susanne M. ; Boddeke, Erik W.G.M. ; Eggen, Bart J.L. / Profiling Microglia From Alzheimer’s Disease Donors and Non-demented Elderly in Acute Human Postmortem Cortical Tissue. I: Frontiers in Molecular Neuroscience. 2020 ; Bind 13.

Bibtex

@article{279f2fd9174e48939bf4b6d4bda60fcf,
title = "Profiling Microglia From Alzheimer{\textquoteright}s Disease Donors and Non-demented Elderly in Acute Human Postmortem Cortical Tissue",
abstract = "Microglia are the tissue-resident macrophages of the central nervous system (CNS). Recent studies based on bulk and single-cell RNA sequencing in mice indicate high relevance of microglia with respect to risk genes and neuro-inflammation in Alzheimer{\textquoteright}s disease (AD). Here, we investigated microglia transcriptomes at bulk and single-cell levels in non-demented elderly and AD donors using acute human postmortem cortical brain samples. We identified seven human microglial subpopulations with heterogeneity in gene expression. Notably, gene expression profiles and subcluster composition of microglia did not differ between AD donors and non-demented elderly in bulk RNA sequencing nor in single-cell sequencing.",
keywords = "Alzheimer{\textquoteright}s disease, barcoded Smart-seq2, human, microglia, single-cell RNA sequencing",
author = "Alsema, {Astrid M.} and Qiong Jiang and Laura Kracht and Emma Gerrits and Dubbelaar, {Marissa L.} and Anneke Miedema and Nieske Brouwer and Hol, {Elly M.} and Jinte Middeldorp and {van Dijk}, Roland and Maya Woodbury and Astrid Wachter and Simon Xi and Thomas M{\"o}ller and Biber, {Knut P.} and Kooistra, {Susanne M.} and Boddeke, {Erik W.G.M.} and Eggen, {Bart J.L.}",
year = "2020",
doi = "10.3389/fnmol.2020.00134",
language = "English",
volume = "13",
journal = "Frontiers in Molecular Neuroscience",
issn = "1662-5099",
publisher = "Frontiers Research Foundation",

}

RIS

TY - JOUR

T1 - Profiling Microglia From Alzheimer’s Disease Donors and Non-demented Elderly in Acute Human Postmortem Cortical Tissue

AU - Alsema, Astrid M.

AU - Jiang, Qiong

AU - Kracht, Laura

AU - Gerrits, Emma

AU - Dubbelaar, Marissa L.

AU - Miedema, Anneke

AU - Brouwer, Nieske

AU - Hol, Elly M.

AU - Middeldorp, Jinte

AU - van Dijk, Roland

AU - Woodbury, Maya

AU - Wachter, Astrid

AU - Xi, Simon

AU - Möller, Thomas

AU - Biber, Knut P.

AU - Kooistra, Susanne M.

AU - Boddeke, Erik W.G.M.

AU - Eggen, Bart J.L.

PY - 2020

Y1 - 2020

N2 - Microglia are the tissue-resident macrophages of the central nervous system (CNS). Recent studies based on bulk and single-cell RNA sequencing in mice indicate high relevance of microglia with respect to risk genes and neuro-inflammation in Alzheimer’s disease (AD). Here, we investigated microglia transcriptomes at bulk and single-cell levels in non-demented elderly and AD donors using acute human postmortem cortical brain samples. We identified seven human microglial subpopulations with heterogeneity in gene expression. Notably, gene expression profiles and subcluster composition of microglia did not differ between AD donors and non-demented elderly in bulk RNA sequencing nor in single-cell sequencing.

AB - Microglia are the tissue-resident macrophages of the central nervous system (CNS). Recent studies based on bulk and single-cell RNA sequencing in mice indicate high relevance of microglia with respect to risk genes and neuro-inflammation in Alzheimer’s disease (AD). Here, we investigated microglia transcriptomes at bulk and single-cell levels in non-demented elderly and AD donors using acute human postmortem cortical brain samples. We identified seven human microglial subpopulations with heterogeneity in gene expression. Notably, gene expression profiles and subcluster composition of microglia did not differ between AD donors and non-demented elderly in bulk RNA sequencing nor in single-cell sequencing.

KW - Alzheimer’s disease

KW - barcoded Smart-seq2

KW - human

KW - microglia

KW - single-cell RNA sequencing

U2 - 10.3389/fnmol.2020.00134

DO - 10.3389/fnmol.2020.00134

M3 - Journal article

C2 - 33192286

AN - SCOPUS:85095957439

VL - 13

JO - Frontiers in Molecular Neuroscience

JF - Frontiers in Molecular Neuroscience

SN - 1662-5099

M1 - 134

ER -

ID: 251940498