Immunohistochemistry, Microscopy, and Image Analysis of Human Muscle Biopsies: Muscle Fiber Denervation as a Working Example

Publikation: Bidrag til bog/antologi/rapport › Bidrag til bog/antologi › Forskning › fagfællebedømt

Standard

Immunohistochemistry, Microscopy, and Image Analysis of Human Muscle Biopsies: Muscle Fiber Denervation as a Working Example. / Soendenbroe, Casper; Andersen, Jesper L.; Mackey, Abigail L.

Neuromuscular Assessments of Form and Function. Humana Press, 2023. s. 145-165 Chapter 9 (Neuromethods, Bind 204).

Publikation: Bidrag til bog/antologi/rapport › Bidrag til bog/antologi › Forskning › fagfællebedømt

Harvard

Soendenbroe, C, Andersen, JL & Mackey, AL 2023, Immunohistochemistry, Microscopy, and Image Analysis of Human Muscle Biopsies: Muscle Fiber Denervation as a Working Example. i Neuromuscular Assessments of Form and Function., Chapter 9, Humana Press, Neuromethods, bind 204, s. 145-165. https://doi.org/10.1007/978-1-0716-3315-1_9

APA

Soendenbroe, C., Andersen, J. L., & Mackey, A. L. (2023). Immunohistochemistry, Microscopy, and Image Analysis of Human Muscle Biopsies: Muscle Fiber Denervation as a Working Example. I Neuromuscular Assessments of Form and Function (s. 145-165). [Chapter 9] Humana Press. Neuromethods Bind 204 https://doi.org/10.1007/978-1-0716-3315-1_9

Vancouver

Soendenbroe C, Andersen JL, Mackey AL. Immunohistochemistry, Microscopy, and Image Analysis of Human Muscle Biopsies: Muscle Fiber Denervation as a Working Example. I Neuromuscular Assessments of Form and Function. Humana Press. 2023. s. 145-165. Chapter 9. (Neuromethods, Bind 204). https://doi.org/10.1007/978-1-0716-3315-1_9

Author

Soendenbroe, Casper ; Andersen, Jesper L. ; Mackey, Abigail L. / Immunohistochemistry, Microscopy, and Image Analysis of Human Muscle Biopsies: Muscle Fiber Denervation as a Working Example. Neuromuscular Assessments of Form and Function. Humana Press, 2023. s. 145-165 (Neuromethods, Bind 204).

Bibtex

@inbook{c237e833f97b4fc882525a950c620a81,
title = "Immunohistochemistry, Microscopy, and Image Analysis of Human Muscle Biopsies: Muscle Fiber Denervation as a Working Example",
abstract = "Immunohistochemistry, fluorescent microscopy, and image analysis are key tools for visualizing and analyzing specific proteins in a variety of tissues. Together these techniques are readily available in most laboratories and in an infinite number of variations. Herein, a working example is used relating to the aging human skeletal muscle being characterized by denervated muscle fibers that undergo atrophy and eventual death. As such, proteins normally restricted to embryonic development, muscle regeneration, and the myotendinous and neuromuscular junctions of the muscle fiber are observed in denervated muscle fibers. We describe a workflow from muscle biopsy sampling to lab bench and to image analysis. Our aim is—through a concrete example—to provide the reader with knowledge and insight into how to design an effective analysis workflow, which can then be utilized in specific research contexts.",
author = "Casper Soendenbroe and Andersen, {Jesper L.} and Mackey, {Abigail L.}",
year = "2023",
doi = "10.1007/978-1-0716-3315-1_9",
language = "English",
isbn = "978-1-0716-3314-4",
series = "Neuromethods",
publisher = "Humana Press",
pages = "145--165",
booktitle = "Neuromuscular Assessments of Form and Function",
address = "United States",

}

RIS

TY - CHAP

T1 - Immunohistochemistry, Microscopy, and Image Analysis of Human Muscle Biopsies: Muscle Fiber Denervation as a Working Example

AU - Soendenbroe, Casper

AU - Andersen, Jesper L.

AU - Mackey, Abigail L.

PY - 2023

Y1 - 2023

N2 - Immunohistochemistry, fluorescent microscopy, and image analysis are key tools for visualizing and analyzing specific proteins in a variety of tissues. Together these techniques are readily available in most laboratories and in an infinite number of variations. Herein, a working example is used relating to the aging human skeletal muscle being characterized by denervated muscle fibers that undergo atrophy and eventual death. As such, proteins normally restricted to embryonic development, muscle regeneration, and the myotendinous and neuromuscular junctions of the muscle fiber are observed in denervated muscle fibers. We describe a workflow from muscle biopsy sampling to lab bench and to image analysis. Our aim is—through a concrete example—to provide the reader with knowledge and insight into how to design an effective analysis workflow, which can then be utilized in specific research contexts.

AB - Immunohistochemistry, fluorescent microscopy, and image analysis are key tools for visualizing and analyzing specific proteins in a variety of tissues. Together these techniques are readily available in most laboratories and in an infinite number of variations. Herein, a working example is used relating to the aging human skeletal muscle being characterized by denervated muscle fibers that undergo atrophy and eventual death. As such, proteins normally restricted to embryonic development, muscle regeneration, and the myotendinous and neuromuscular junctions of the muscle fiber are observed in denervated muscle fibers. We describe a workflow from muscle biopsy sampling to lab bench and to image analysis. Our aim is—through a concrete example—to provide the reader with knowledge and insight into how to design an effective analysis workflow, which can then be utilized in specific research contexts.

U2 - 10.1007/978-1-0716-3315-1_9

DO - 10.1007/978-1-0716-3315-1_9

M3 - Book chapter

SN - 978-1-0716-3314-4

T3 - Neuromethods

SP - 145

EP - 165

BT - Neuromuscular Assessments of Form and Function

PB - Humana Press

ER -

ID: 360976396