Histone H2A C-terminus regulates chromatin dynamics, remodeling, and histone H1 binding
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Histone H2A C-terminus regulates chromatin dynamics, remodeling, and histone H1 binding. / Vogler, Christine; Huber, Claudia; Waldmann, Tanja; Ettig, Ramona; Braun, Lora; Izzo, Annalisa; Daujat, Sylvain; Chassignet, Isabelle; Lopez-Contreras, Andres Joaquin; Fernandez-Capetillo, Oscar; Dundr, Miroslav; Rippe, Karsten; Längst, Gernot; Schneider, Robert.
I: P L o S Genetics, Bind 6, Nr. 12, 2010, s. e1001234.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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TY - JOUR
T1 - Histone H2A C-terminus regulates chromatin dynamics, remodeling, and histone H1 binding
AU - Vogler, Christine
AU - Huber, Claudia
AU - Waldmann, Tanja
AU - Ettig, Ramona
AU - Braun, Lora
AU - Izzo, Annalisa
AU - Daujat, Sylvain
AU - Chassignet, Isabelle
AU - Lopez-Contreras, Andres Joaquin
AU - Fernandez-Capetillo, Oscar
AU - Dundr, Miroslav
AU - Rippe, Karsten
AU - Längst, Gernot
AU - Schneider, Robert
PY - 2010
Y1 - 2010
N2 - The tails of histone proteins are central players for all chromatin-mediated processes. Whereas the N-terminal histone tails have been studied extensively, little is known about the function of the H2A C-terminus. Here, we show that the H2A C-terminal tail plays a pivotal role in regulating chromatin structure and dynamics. We find that cells expressing C-terminally truncated H2A show increased stress sensitivity. Moreover, both the complete and the partial deletion of the tail result in increased histone exchange kinetics and nucleosome mobility in vivo and in vitro. Importantly, our experiments reveal that the H2A C-terminus is required for efficient nucleosome translocation by ISWI-type chromatin remodelers and acts as a novel recognition module for linker histone H1. Thus, we suggest that the H2A C-terminal tail has a bipartite function: stabilisation of the nucleosomal core particle, as well as mediation of the protein interactions that control chromatin dynamics and conformation.
AB - The tails of histone proteins are central players for all chromatin-mediated processes. Whereas the N-terminal histone tails have been studied extensively, little is known about the function of the H2A C-terminus. Here, we show that the H2A C-terminal tail plays a pivotal role in regulating chromatin structure and dynamics. We find that cells expressing C-terminally truncated H2A show increased stress sensitivity. Moreover, both the complete and the partial deletion of the tail result in increased histone exchange kinetics and nucleosome mobility in vivo and in vitro. Importantly, our experiments reveal that the H2A C-terminus is required for efficient nucleosome translocation by ISWI-type chromatin remodelers and acts as a novel recognition module for linker histone H1. Thus, we suggest that the H2A C-terminal tail has a bipartite function: stabilisation of the nucleosomal core particle, as well as mediation of the protein interactions that control chromatin dynamics and conformation.
KW - Amino Acid Motifs
KW - Cell Line
KW - Chromatin
KW - Chromatin Assembly and Disassembly
KW - Histones
KW - Humans
KW - Nucleosomes
KW - Protein Binding
U2 - 10.1371/journal.pgen.1001234
DO - 10.1371/journal.pgen.1001234
M3 - Journal article
C2 - 21170357
VL - 6
SP - e1001234
JO - P L o S Genetics
JF - P L o S Genetics
SN - 1553-7390
IS - 12
ER -
ID: 135740913