Preserved stem cell content and innervation profile of elderly human skeletal muscle with lifelong recreational exercise
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Preserved stem cell content and innervation profile of elderly human skeletal muscle with lifelong recreational exercise. / Soendenbroe, Casper; Dahl, Christopher L.; Meulengracht, Christopher; Tamáš, Michal; Svensson, Rene B.; Schjerling, Peter; Kjaer, Michael; Andersen, Jesper L.; Mackey, Abigail L.
In: The Journal of Physiology, Vol. 600, No. 8, 2022, p. 1969-1989.Research output: Contribution to journal › Journal article › Research › peer-review
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T1 - Preserved stem cell content and innervation profile of elderly human skeletal muscle with lifelong recreational exercise
AU - Soendenbroe, Casper
AU - Dahl, Christopher L.
AU - Meulengracht, Christopher
AU - Tamáš, Michal
AU - Svensson, Rene B.
AU - Schjerling, Peter
AU - Kjaer, Michael
AU - Andersen, Jesper L.
AU - Mackey, Abigail L.
PY - 2022
Y1 - 2022
N2 - Muscle fibre denervation and declining numbers of muscle stem (satellite) cells are defining characteristics of ageing skeletal muscle. The aim of this study was to investigate the potential for lifelong recreational exercise to offset muscle fibre denervation and compromised satellite cell content and function, both at rest and under challenged conditions. Sixteen elderly lifelong recreational exercisers (LLEX) were studied alongside groups of age-matched sedentary (SED) and young subjects. Lean body mass and maximal voluntary contraction were assessed, and a strength training bout was performed. From muscle biopsies, tissue and primary myogenic cell cultures were analysed by immunofluorescence and RT-qPCR to assess myofibre denervation and satellite cell quantity and function. LLEX demonstrated superior muscle function under challenged conditions. When compared with SED, the muscle of LLEX was found to contain a greater content of satellite cells associated with type II myofibres specifically, along with higher mRNA levels of the beta and gamma acetylcholine receptors (AChR). No difference was observed between LLEX and SED for the proportion of denervated fibres or satellite cell function, as assessed in vitro by myogenic cell differentiation and fusion index assays. When compared with inactive counterparts, the skeletal muscle of lifelong exercisers is characterised by greater fatigue resistance under challenged conditions in vivo, together with a more youthful tissue satellite cell and AChR profile. Our data suggest a little recreational level exercise goes a long way in protecting against the emergence of classic phenotypic traits associated with the aged muscle.
AB - Muscle fibre denervation and declining numbers of muscle stem (satellite) cells are defining characteristics of ageing skeletal muscle. The aim of this study was to investigate the potential for lifelong recreational exercise to offset muscle fibre denervation and compromised satellite cell content and function, both at rest and under challenged conditions. Sixteen elderly lifelong recreational exercisers (LLEX) were studied alongside groups of age-matched sedentary (SED) and young subjects. Lean body mass and maximal voluntary contraction were assessed, and a strength training bout was performed. From muscle biopsies, tissue and primary myogenic cell cultures were analysed by immunofluorescence and RT-qPCR to assess myofibre denervation and satellite cell quantity and function. LLEX demonstrated superior muscle function under challenged conditions. When compared with SED, the muscle of LLEX was found to contain a greater content of satellite cells associated with type II myofibres specifically, along with higher mRNA levels of the beta and gamma acetylcholine receptors (AChR). No difference was observed between LLEX and SED for the proportion of denervated fibres or satellite cell function, as assessed in vitro by myogenic cell differentiation and fusion index assays. When compared with inactive counterparts, the skeletal muscle of lifelong exercisers is characterised by greater fatigue resistance under challenged conditions in vivo, together with a more youthful tissue satellite cell and AChR profile. Our data suggest a little recreational level exercise goes a long way in protecting against the emergence of classic phenotypic traits associated with the aged muscle.
U2 - 10.1113/JP282677
DO - 10.1113/JP282677
M3 - Journal article
C2 - 35229299
VL - 600
SP - 1969
EP - 1989
JO - The Journal of Physiology
JF - The Journal of Physiology
SN - 0022-3751
IS - 8
ER -
ID: 298603516