Aging is associated with an altered macrophage response during human skeletal muscle regeneration

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Standard

Aging is associated with an altered macrophage response during human skeletal muscle regeneration. / Ahmadi, Mohadeseh; Karlsen, Anders; Mehling, Jack; Soendenbroe, Casper; Mackey, Abigail L.; Hyldahl, Robert D.

I: Experimental Gerontology, 2022.

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt

Harvard

Ahmadi, M, Karlsen, A, Mehling, J, Soendenbroe, C, Mackey, AL & Hyldahl, RD 2022, 'Aging is associated with an altered macrophage response during human skeletal muscle regeneration', Experimental Gerontology. https://doi.org/10.1016/j.exger.2022.111974

APA

Ahmadi, M., Karlsen, A., Mehling, J., Soendenbroe, C., Mackey, A. L., & Hyldahl, R. D. (2022). Aging is associated with an altered macrophage response during human skeletal muscle regeneration. Experimental Gerontology, [111974]. https://doi.org/10.1016/j.exger.2022.111974

Vancouver

Ahmadi M, Karlsen A, Mehling J, Soendenbroe C, Mackey AL, Hyldahl RD. Aging is associated with an altered macrophage response during human skeletal muscle regeneration. Experimental Gerontology. 2022. 111974. https://doi.org/10.1016/j.exger.2022.111974

Author

Ahmadi, Mohadeseh ; Karlsen, Anders ; Mehling, Jack ; Soendenbroe, Casper ; Mackey, Abigail L. ; Hyldahl, Robert D. / Aging is associated with an altered macrophage response during human skeletal muscle regeneration. I: Experimental Gerontology. 2022.

Bibtex

@article{0ce3deee58f549d692b3cdbc4ddcd661,
title = "Aging is associated with an altered macrophage response during human skeletal muscle regeneration",
abstract = "Skeletal muscle injury in aged rodents is characterized by an asynchronous infiltration of pro- and anti-inflammatory macrophage waves, leading to improper and incomplete regeneration. It is unclear whether this aberration also occurs in aged human muscle. In this study, we quantified the macrophage responses in a human model of muscle damage and regeneration induced by electrical stimulation in 7 young and 21 older adults. At baseline, total resident macrophage (CD68+/DAPI+) content was not different between young and old subjects, but pro-inflammatory (CD206−/CD68+/DAPI+) macrophage content was lower in the old. Following damage, muscle Infiltration of CD206−/CD68+/DAPI+ macrophages was lower in old relative to young subjects. Further, only the increase in CD206−/CD68+ macrophages correlated with the change in muscle satellite cell content. Our data show that older individuals have a compromised macrophage response during muscle regeneration, pointing to an altered inflammatory response as a potential mechanism for reduced muscle regenerative efficacy in aged humans.",
author = "Mohadeseh Ahmadi and Anders Karlsen and Jack Mehling and Casper Soendenbroe and Mackey, {Abigail L.} and Hyldahl, {Robert D.}",
year = "2022",
doi = "10.1016/j.exger.2022.111974",
language = "English",
journal = "Experimental Gerontology",
issn = "0531-5565",
publisher = "Elsevier",

}

RIS

TY - JOUR

T1 - Aging is associated with an altered macrophage response during human skeletal muscle regeneration

AU - Ahmadi, Mohadeseh

AU - Karlsen, Anders

AU - Mehling, Jack

AU - Soendenbroe, Casper

AU - Mackey, Abigail L.

AU - Hyldahl, Robert D.

PY - 2022

Y1 - 2022

N2 - Skeletal muscle injury in aged rodents is characterized by an asynchronous infiltration of pro- and anti-inflammatory macrophage waves, leading to improper and incomplete regeneration. It is unclear whether this aberration also occurs in aged human muscle. In this study, we quantified the macrophage responses in a human model of muscle damage and regeneration induced by electrical stimulation in 7 young and 21 older adults. At baseline, total resident macrophage (CD68+/DAPI+) content was not different between young and old subjects, but pro-inflammatory (CD206−/CD68+/DAPI+) macrophage content was lower in the old. Following damage, muscle Infiltration of CD206−/CD68+/DAPI+ macrophages was lower in old relative to young subjects. Further, only the increase in CD206−/CD68+ macrophages correlated with the change in muscle satellite cell content. Our data show that older individuals have a compromised macrophage response during muscle regeneration, pointing to an altered inflammatory response as a potential mechanism for reduced muscle regenerative efficacy in aged humans.

AB - Skeletal muscle injury in aged rodents is characterized by an asynchronous infiltration of pro- and anti-inflammatory macrophage waves, leading to improper and incomplete regeneration. It is unclear whether this aberration also occurs in aged human muscle. In this study, we quantified the macrophage responses in a human model of muscle damage and regeneration induced by electrical stimulation in 7 young and 21 older adults. At baseline, total resident macrophage (CD68+/DAPI+) content was not different between young and old subjects, but pro-inflammatory (CD206−/CD68+/DAPI+) macrophage content was lower in the old. Following damage, muscle Infiltration of CD206−/CD68+/DAPI+ macrophages was lower in old relative to young subjects. Further, only the increase in CD206−/CD68+ macrophages correlated with the change in muscle satellite cell content. Our data show that older individuals have a compromised macrophage response during muscle regeneration, pointing to an altered inflammatory response as a potential mechanism for reduced muscle regenerative efficacy in aged humans.

U2 - 10.1016/j.exger.2022.111974

DO - 10.1016/j.exger.2022.111974

M3 - Journal article

C2 - 36228835

JO - Experimental Gerontology

JF - Experimental Gerontology

SN - 0531-5565

M1 - 111974

ER -

ID: 321945958